2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-147339
    M7G(3'-OMe-5')pppA(2'-OMe)
    M7G(3'-OMe-5')pppA(2'-OMe) is a cap analogue for mRNA synthesis in vitro.
    M7G(3'-OMe-5')pppA(2'-OMe)
  • HY-18572S1
    2,4-D-d3
    2,4-D-d3 is the deuterium labeled 2,4-D. 2,4-D (2,4-Dichlorophenoxyacetic acid) is a selective systemic herbicide for the control of broad-leaved weeds. 2,4-D acts as a plant hormone, causing uncontrolled growth in the meristematic tissues. 2,4-D inhibits DNA and protein synthesis and thereby prevents normal plant growth and development.
    2,4-D-d<sub>3</sub>
  • HY-149877
    hDHODH-IN-12
    		Inhibitor
    hDHODH-IN-12 is a potent DHODH inhibitor with an IC50 value of 0.421 μM. DHODH is the rate-limiting enzyme in the de novo synthesis of pyrimidine which is essential in DNA/RNA Synthesis. hDHODH-IN-12 is present in the inner membrane of human mitochondria.hDHODH-IN-12 can be used for the research of lung cancer.
    hDHODH-IN-12
  • HY-122157
    YM-534
    YM-534, an imidazole compound, is an anti-cancer agent. YM-534 inhibits HL-60 cells with an IC50 of 2.5 μM. YM-534 retards the processing of preribosomal to ribosomal RNA of HL-60 cells. YM-534 can be used for the study of promyelocytic leukemia.
    YM-534
  • HY-158675
    3'-P-dCTP sodium
    3'-P-dCTP sodium is a labeled modified deoxyoligonucleotide (dNTP) that can release pyrophosphate to produce fluorescence and has special applications in gene synthesis and sequencing.
    3'-P-dCTP sodium
  • HY-162603
    HDAC-IN-74
    		Inhibitor
    HDAC-IN-74 (PA) is a dual HDAC/Rribonucleotide reductase(RR) inhibitor, with IC50 values ​​of 10.80 μM and 9.34 μM for HDAC and HDAC, respectively. HDAC-IN-74 can be used in anticancer research.
    HDAC-IN-74
  • HY-E70086A
    Taq DNA Polymerase, Glycerol-free
    Taq DNA Polymerase, Glycerol-free is a thermostable DNA polymerase that can be used in PCR.
    Taq DNA Polymerase, Glycerol-free
  • HY-W042357S3
    Ac-rC Phosphoramidite-13C2,d1
    Ac-rC Phosphoramidite-13C2,d1 is deuterium and 13C-labeled Ac-rC Phosphoramidite (HY-W042357). Ac-rC Phosphoramidite is used for the oligoribonucleotide phosphorodithioate modification (PS2-RNA).
    Ac-rC Phosphoramidite-<sup>13</sup>C<sub>2</sub>,d<sub>1</sub>
  • HY-161617
    LASSBio-2052
    		Inhibitor
    LASSBio-2052 is a derivative of N-acylhydrazone with antitumor activity against hepatocellular carcinoma (HCC). LASSBio-2052 inhibits HCC cells HepG2 and Hep3B, with IC50 of 18 and 41 μM. LASSBio-2052 arrests the cell cycle at G2/M phase, through downregulation of FOXM1. LASSBio-2052 induces apoptosis in HCC cells.
    LASSBio-2052
  • HY-163892
    POLRMT-IN-1
    		Inhibitor
    POLRMT-IN-1 (compound S7) is a POLRMT inhibitor. POLRMT-IN-1 can be used in cancer-related research.
    POLRMT-IN-1
  • HY-173455
    NP1867
    		Inhibitor
    NP1867 is a potent, selective, covalent PMS2 inhibitor. NP1867 functionally inhibits DNA mismatch repair. NP1867 enhances immune surveillance. NP1867 can be used in the research of colorectal cancer.
    NP1867
  • HY-N1377R
    Nevadensin (Standard)
    		Inhibitor
    Nevadensin (Standard) (Lysionotin (Standard)) is the analytical standard of Nevadensin (HY-N1377). This product is intended for research and analytical applications. Nevadensin, a natural flavonoid, is a selective human carboxylesterase 1 (hCE1) inhibitor with an IC50 of 2.64 μM. Nevadensin is more selective for hCE1 than hCE2 (IC50 of 132.8 μM). Nevadensin can induce apoptosis and DNA damage in cancer cells. Nevadensin has a variety of pharmacological effects such as anti-inflammatory, anti-tumour, anti-hypertensive, anti-tubercular, antitussive, antioxidant and anti-microbial activities.
    Nevadensin (Standard)
  • HY-N14021
    Petasites japonicus extract
    		Activator
    Petasites japonicus extract is the extract from Petasites japonicus with antioxidant, anti-inflammatory, anti-obesity, neuroprotective and anti-cancer effects. Petasites japonicus extract promotes osteoblast differentiation via up-regulation of Runx2 and Osterix in MC3T3-E1 cells.
    Petasites japonicus extract
  • HY-137697B
    ddCTP trilithium
    		Inhibitor
    ddCTP trilithium is one of 2',3'-dideoxyribonucleoside 5'-triphosphates (ddNTPs) that acts as chain-elongating inhibitor of DNA polymerase for DNA sequencing. ddCTP trilithium is a nucleoside analog that targets the reverse trsanscriptase of human immunodeficiency virus (HIV). ddCTP trilithium can be used for AIDS research.
    ddCTP trilithium
  • HY-149379
    DNA gyrase B-IN-3
    		Inhibitor
    DNA gyrase B-IN-3 (Compound A) is a bacterial DNA gyrase B inhibitor (IC50: < 10 nM). DNA gyrase B-IN-3 has antibacterial activity against gram-positive strains.
    DNA gyrase B-IN-3
  • HY-148384A
    UHMCP1 dihydrochloride
    		Inhibitor
    UHMCP1 dihydrochloride is a potent UHM domain splicing inhibitor with a Kd value of 79 μM. UHMCP1 dihydrochloride prevents the SF3b155/U2AF65 interaction. UHMCP1 dihydrochloride impacts cell viability and RNA splicing.
    UHMCP1 dihydrochloride
  • HY-162896
    And1 degrader 1
    		Degrader
    And1 degrader 1 (Compound A15) is a degrader of the acidic nucleoplasmic DNA-binding protein 1 (And1), which can significantly induce the degradation of And1 in NSCLC cells. And1 degrader 1 (5 μM) combined with Olaparib (HY-10162) (1 μM) effectively inhibits the proliferation of A549 and H460 cells. And1 degrader 1 can be used in cancer research.
    And1 degrader 1
  • HY-W013068S3
    DMT-dT Phosphoramidite-d11
    DMT-dT Phosphoramidite-d11 is deuterium labeled DMT-dT Phosphoramidite (HY-W013068). DMT-dT Phosphoramidite is typically used in the synthesis of DNA.
    DMT-dT Phosphoramidite-d<sub>11</sub>
  • HY-163882
    CUHK242
    		Inhibitor
    CUHK242 is a bacterial transcription inhibitor, with a MIC of 2 μg/mL for B. subtilis reporter strain BS2019. CUHK242 has antimicrobial activity against Staphylococcus aureus. CUHK242 can inhibit RNA synthesis in cells, thereby simultaneously reducing protein synthesis.
    CUHK242
  • HY-116692
    Apoptosis inducer 34
    		Inhibitor
    Apoptosis inducer 34 (Compound 4) is a small molecule compound that induces apoptosis by directly activating the intrinsic apoptotic pathway. Apoptosis inducer 34 promotes Apaf-1 oligomerization to form mature apoptosomes, thereby activating caspase-9 and caspase-3. It significantly activates the apoptotic pathway in Jurkat cells by enhancing the cytochrome c-dependent apoptotic signaling pathway, inducing PARP cleavage and chromosomal DNA fragmentation. Furthermore, Apoptosis inducer 34 exhibits low toxicity to normal cells, demonstrating potential for selective targeting of cancer cells. Apoptosis inducer 34 is a promising candidate for studying cancer related to apoptotic pathways.
    Apoptosis inducer 34
Cat. No. Product Name / Synonyms Application Reactivity